Brain mechanics controls neuronal growth
Keynote lecture: Thursday September 28, 9:00-9:45 am
Dr. Kristian Franze – Department of Physiology, Development and Neuroscience, University of Cambridge
During development and pathological processes, cells in the central nervous system (CNS) are highly motile. Despite the fact that cell motion is driven by forces, our current understanding of the mechanical interactions between CNS cells and their environment is very limited. We here show how nanometer deformations of CNS tissue caused by piconewton forces exerted by cells contribute to regulating CNS development and pathologies. In vitro, growth and migration velocities, directionality, cellular forces as well as neuronal fasciculation and maturation all significantly depended on substrate stiffness. Moreover, when grown on substrates incorporating linear stiffness gradients, glial cells migrated towards stiffer, while axon bundles turned towards softer substrates. In vivo atomic force microscopy revealed stiffness gradients in developing brain tissue, which axons followed as well towards soft. Interfering with brain stiffness and mechanosensitive ion channels in vivo both led to similar aberrant neuronal growth patterns with reduced fasciculation and pathfinding errors. Importantly, CNS tissue significantly softened after traumatic injuries. Ultimately, mechanical signals not only directly impacted neuronal growth but also indirectly by regulating neuronal responses to chemical guidance cues, strongly suggesting that neuronal growth is not only controlled by chemical signals – as it is currently assumed – but also by the tissue’s local physical properties.